CAT N° : S175T, S180T, S181T, S182T Collected from the source : When searchers choose their serum an important factor that should be taken into consideration is the source, which also emphasises the traceability of the serum. Our system of vertical integration allows us to be certain of the origins and traceability of our FBS. Each manufactured batch is rigorously controlled, from the collection of serum and throughout all stages of its treatment and production through to final packaging on our premises. BioWest Fetal Bovine Serum is derived from clotted whole blood aseptically collected from fetus via cardiac puncture. The serum is collected or imported and treated in agreement with the European regulations. Filtration : Final Filter Size :
All sera are tested for the absence of aerobic and anaerobic bacteria, fungi, yeast and Mycoplasma. The sterility test is based on the European Pharmacopoeia requirements. The sera are tested for the absence of Mycoplasma by culture. Virus Tested : All of our serum is tested for: -
Sera are tested for the absence of the indicated viruses by inoculation to GBK cells. The revelation is made by indirect immunofluorescence. The antibodies are tested by an Elisa method. Endotoxin : All sera are tested to determine the levels of endotoxins. BioWest carries out a chromokinetic quantitative test, according to the method D of the European Pharmacopoeia. The endotoxin reagent is standardized against the US reference endotoxin. Haemoglobin : The haemoglobin level is measured by spectrophotometer. Osmolality :
Determined by a lowered freezing temperature. The osmometer is calibrated against standard solutions Cell Culture : Biological performance is assessed using cell culture medium supplemented with the serum being tested. During the test period, cultures are examined microscopically for any morphological abnormalities that may indicate toxic components in the serum. Cell Culture Tests :
Cell Growth, Plating Efficiency, Cloning Efficiency.
Cell Lines Tested :
The following cell lines are tested with the serum:
SP2/O-AG14 -Mouse/Lymphoma MRC- 5 -Human/Lung.
Total Protein : Determined by Biuret Colorimetry. Tetracycline test : BioWest delegate this test to an independent laboratory. The laboratory uses a liquid chromatography electrospray ionisation tandem mass spectrometry method to detect the Tetatrycline. This method allows also the detection of the Chlortetracycline and the Oxytetracycline in the same time. The limit de detection is 0.05 mg/l. Country of Origin : The country in which the serum was taken from the donor/animal. BioWest sera are sourced from the following countries Cat N° S175T Denmark
Cat N° S181T South America Cat N° S182T France
Storage conditions : - 20°C
Shelf life : 5 years
Recommended use : - Respect storage conditions of the serum - Do not use the serum after its expiry date - Store serum in an area protected from light - Manipulate serum in aseptic conditions (e.g. : under laminar air flow) - Wear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…) - In order to preserve all serum qualities, it is recommended to thaw out the flask, to aliquote, then to re-freeze the produced flasks rather than to thaw out and re-freeze the flask at each use. - It is recommended to use the serum immediately after its thaw out. However, if it is not useful, it is possible to store thaw out serum, at +2°C / +8°C, until 26 weeks without significant decrease of its performances in cell culture. The product is intended to be used in vitro, in laboratory only. Do not use it in therapy, human or veterinary applications.
FEATURE ARTICLE B A R C N E W S L E T T E R Manish Goswami, Suhas H. Mangoli and Narendra JawaliDifferent types of biochemical and physiological factors (including commonly used cellular anddietary antioxidants) affect the activity of therapeutic antibiotics. The findings from our lab haveshown that the presence of glutathione, N-acetylcysteine or ascorbic acid in the growth mediumdecreases
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