Technical Bulletin Delvotest® SP Test Sensitivity or Detection levels Introduction Delvotest® SP broad-spectrum antibiotic residue test system offers the possibility to verify the presence in milk of a multitude of different antimicrobial substances. Delvotest® SP may be used in milk quality payment programs. An indication of the sensitivity of the Delvotest® SP has been given in the Specification sheet of this product. However, apart from the specified sensitivities for Penicillin-G and Sulfadiazine, a substantial number of other antibiotics, sulfonamides and inhibitory substances can be detected at or close to the levels such as defined by the Maximum Residue Level (MRL). The table below shows an overview of the indicative detection levels of Delvotest® SP for different antibiotic residues.
Delvotest® SP indicative data on detection levels Delvotest® SP in ppb (parts per billion or ng/ml) in commingled rawcow’s milk. Control time Fixed reading time Time of negative control Between 2 hours, 45 minutes Colouring yellow and 3 hours ß-lactams Penicillin-G Sulphonamides Sulfadiazine Tetracyclines Tetracycline Macrolides Erythromycin Aminoglycosides Gentamycin Lincosamides Lincomycin Others Chloramphenicol Technical Bulletin Delvotest® SP Test Sensitivity or Detection levels
The data indicated in the table are based upon readings judged as being yellow/purple to purple and are to beregarded as an indication. It is recommended to always confirm positive test results. Variations in incubation times andtemperatures, operators and storage conditions may have an influence on the reading result found. Data in the tablemay be used for validation purposes. Definitions Drugs:
Active ingredient of cattle medication such as antibiotics and sulfonamides.
An indication of the number of different active ingredients that can be detected by agiven method at or below the required detection level.
The lowest concentration at which a drug is still detected by the test, especially atcontrol time and reading time.
Time at which the test agar containing the negative control sample has justchanged to yellow. At this time the best sensitivity for the test system is obtained.
For use without the requirement of running a negative control sample a fixedreading time between 2 hours 45 minutes and 3 hours can be used. This maydecrease the sensitivity for bacteriostatic drugs.
Not sensitive, detection limit above 10000 ppb (ng/ml) (10
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